Vincent Noireaux
Libchaber Lab, Center for Studies in Physics and Biology, Rockefeller University.
| A cell-free expression system has been used as a bottom-up approach toward the engineering of a minimal living cell.Cell-free genetic circuit elements were constructed in a transcription-translation extract. We engineered transcriptional activation and repression cascades, in which the protein product of each stage is the input required to drive or block the following stage. Although we can find regions of linear response for single stages, cascading to subsequent stages requires working in non-linear regimes. Substantial time delays and dramatic decreases in output production are incurred with each additional stage, due to a bottleneck at the translation machinery. Faster turnover of RNA message can relieve competition between genes and stabilize output against variations in input and parameters. |
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